Current Projects

• The in vivo visulization of RGC in mice 
  Submitted by Waiying Li  (The Chinese University of Hong Kong, Hong Kong, Hong Kong) 5/24/2010
  To in vivo visulize the RGC shrinkage in mice expressing YFP in RGC under the control of THY1 promoter.



• Medullary cords in Lymph Nodes 
  Submitted by David Fooksman  (NYU, New York, United States) 5/17/2010
  Medullary cords of lymph nodes contain various phagocytic cells. We want to assess the consequences of depletion of these cells on B lymphocyte migration in the lymph node using intravital microscopy.



• Macrophages and GVHD 
  Submitted by Emanuela Sega  (Stanford University, Stanford, CA, United States) 5/17/2010
  Clodronate liposomes will be used to assess the role of macrophages in amplification of inflammatory cytokines during GVHD development.



• macrophage after toxoplasma gondii infection 
  Submitted by Ying Fang Liao  (UC Berkeley, Berkeley, United States) 11/5/2010
  behavior of macrophage after toxoplasma gondii infection



• Role of glycoproteins in macrophage function  
  Submitted by Eric Park  (University of North Carolina, Chapel Hill, United States) 11/5/2010
  We propose administering the clodronate liposomes ip in our mouse model of IBD to determine if glycoproteins have a role in macrophage function in inflammation. Our preliminary data suggests changes in glycoprotein concentrations alter the number of macrophages in the lamina propria of the colon. # Determine te role of macrophages in Inflammatory Bowel Disease Submitted by Nancy Taylor (Mass Inst Tech, Cambridge, MA, United We propose administering the clodronate liposomes ip in our mouse model of IBD to determine if macrophages play a role in inflamation. This has been suggested in Balb/c mice using the DSS model where a marked infiltration of macrophages in the lamina propria of the colon occurs in the disease.



• The role of inflammation in the activation and contribution of endothelial progenitors in muscle regeneration 
  Submitted by Paola Zordan  (San Raffaele Scientific Institute, Milano, Italy) 11/5/2010
  We are studing, in vivo, the putative crosstalk between Vascular associated progenitors (VAPs) and the macrophage component: with the depletion of all or specific macrophages in combination with the VE-Cad::CreER-mice, we will be able to study the interaction with monocyte/macrophage subpopulations and with gain of function assays we will study the signaling pathways in promoting stem cells activation and recruitment during tissue damage/repair in different models of muscle damage.



• ACE 10/10 mice/Bernstein Lab 
  Submitted by Brian Taylor  (Cedars-Sinai Medical Center, Los Angeles, CA, United States) 5/7/2010
  We'd like to try using it for depletion of macrophages in a line of mice that genetically over-express Angiotensin Converting Enzyme.
  More: http://research.csmc.edu/acad/gradprogram/mentors/profile/1762826



• aortic ring angiogenesis  
  Submitted by Alfred Aplin  (University of Washington, Seattle, WA, United States) 5/5/2010
  Growth of blood vessels from clodronate treated rat aortic rings



• Galectin-3 regulation of lung tumour growth 
  Submitted by Eleni Kouverianou  (University of Edinburgh, Edinburgh, United Kingdom) 4/29/2010
  The project focuses on macrophage influence on tumour growth. We have previously used other means of knocking out macrophages and would like to repeat our study using clodronate liposomes. We would also like to use the compound for increased efficiency in a bone marrow transplant model, as macrophages can be resistant to irradiation. Clodronate liposome administration prior to irradiation and transplant would provide a cleaner model of macrophage transfer.



• Role of Kupffer cells in fibrosis 
  Submitted by Nicolas Lanthier  (Laboratory of Gastroenterology, Brussels, Belgium) 4/29/2010
  We want to study the role of Kupffer cells in models of chronic liver injury, using injections of liposomes encapsulated clodronate.